服務(wù)熱線
17317196276
歡迎您,來到默瑞(上海)生物科技有限公司!
PROUCTS LIST
ExoClean and ExoTracker
K-4300a, K-4300b, K-4300c, K-4300d (20 tests)
Description: Simple, quick protocols for labeling (ExoTracker) and isolating (ExoClean) extracellular vesicles (EVs) from biological samples*
Echelon’s ExoClean – EV Purification Kit and ExoTracker kits為標(biāo)記和純化外顯體和細胞外囊泡(EV)提供了一個簡單可靠的系統(tǒng)。(1)The ExoClean – EV Purification Kit (貨號:K-4300a )通過將沉淀試劑與微離心柱配對,取代了與基于超離心的純化相關(guān)的時間和設(shè)備成本,此外可以去除與其他沉淀技術(shù)相關(guān)的有害污染物。
(2)The ExoTracker kits (貨號:K-4300b -d) 使該系統(tǒng)的實用性更進一步,可以用膜或RNA特異性標(biāo)簽標(biāo)記EV。
產(chǎn)品列表
Catalog # | Description | Quantity |
ExoClean (K-4300a)
K-4301 | EV Precipitation Reagent | 30 mL |
K-SPIN | Microcentrifuge Columns | 20 columns |
ExoTracker - Membrane (K-4300b)
K-4300a | ExoClean | 1 kit |
K-4302 | EV Membrane Label | 150 µg |
ExoTracker - RNA (K-4300c)
K-4300a | ExoClean | 1 kit |
K-4303 | EV RNA Label | 20 µL |
ExoTracker - Total (K-4300d)
K-4300a | ExoClean | 1 kit |
K-4302 | EV Membrane Label | 150 µg |
K-4303 | EV RNA Label | 20 µL |
操作流程:
1:Collect EV sample
2:Centrifuge at 2000g for 10 min to remove any debris or cells.
3:Transfer supernatant and precipitate cleared sample with-K4301 for 30 minutes at room temperature with mixing
4:Centrifuge precipitated sample at 1500g for 30 minutes at room temperature.
5:Discard the supernatant fraction and resuspend the precipitated pellet in 1x PBS.
6:Apply the resuspended pellet to the equilibrated K-SPINcolumn and incubate for 5 minutes at room temperature.
7:Centrifuge the column at 500g for 30 seconds at room temperature.
8:Collect and examine the eluted, purified EVs.
注:Exosomes and extracellular vesicles (EVs)-胞外體和胞外小泡
發(fā)表文獻:
1. Konoshenko MY, Lekchnov EA, Vlassov AV, Laktionov PP. Isolation of Extracellular Vesicles: General Methodologies and Latest Trends. Biomed Res Int. 2018;2018:8545347.
2. Li P, Kaslan M, Lee SH, Yao J, Gao Z. Progress in Exosome Isolation Techniques. Theranostics. 2017;7(3):789-804.
3. Deregibus MC, Figliolini F, D’Antico S, Manzini PM, Pasquino C, De Lena M, et al. Charge-based precipitation of extracellular vesicles. Int J Mol Med. 2016;38(5):1359-66.
默瑞生物:
默瑞(上海)生物科技有限公司,作為專業(yè)的生命科學(xué)產(chǎn)品推廣與服務(wù)商,以“創(chuàng)新,專業(yè),共贏”的理念,不斷強化服務(wù)體系,提升客戶體驗,以莫納生物產(chǎn)品為主線,結(jié)合國內(nèi)外優(yōu)質(zhì)產(chǎn)品提供高標(biāo)準(zhǔn)完整解決方案,幫助科研客戶和生物技術(shù)企業(yè)高效創(chuàng)新,加速轉(zhuǎn)化,創(chuàng)造更多優(yōu)質(zhì)產(chǎn)品,提升社會福祉,實現(xiàn)多方共贏。
默瑞生物創(chuàng)立以來,已經(jīng)陸續(xù)導(dǎo)入TwistDx,博日
Jackson,izon,Biontex,Immunostep,101Bio,enzymatics,AATBioquest,KAPABIOSYSTEMS,BioDynami,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等優(yōu)質(zhì)品牌,逐步培育成為專業(yè)用戶的選擇。